Uncoupled human flavin-containing monooxygenase 3 releases superoxide radical in addition to hydrogen peroxide
نویسندگان
چکیده
منابع مشابه
Mammalian flavin-containing monooxygenase (FMO) as a source of hydrogen peroxide.
Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and 3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂ upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production. Two common FMO2 (the major isoform in the lung) genetic polymorphisms, S19...
متن کاملBacterial flavin-containing monooxygenase is trimethylamine monooxygenase.
Flavin-containing monooxygenases (FMOs) are one of the most important monooxygenase systems in Eukaryotes and have many important physiological functions. FMOs have also been found in bacteria; however, their physiological function is not known. Here, we report the identification and characterization of trimethylamine (TMA) monooxygenase, termed Tmm, from Methylocella silvestris, using a combin...
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Busulphan (Bu) is an alkylating agent used in the conditioning regimen prior to hematopoietic stem cell transplantation (HSCT). Bu is extensively metabolized in the liver via conjugations with glutathione to form the intermediate metabolite (sulfonium ion) which subsequently is degraded to tetrahydrothiophene (THT). THT was reported to be oxidized forming THT-1-oxide that is further oxidized to...
متن کاملRegulation of Flavin-Containing Monooxygenase
The flavin-containing monooxygenases (FMOs) are important for the oxidation of a variety of environmental toxicants, natural products, and therapeutics. Consisting of six family members (FMO1–5), these enzymes exhibit distinct but broad and overlapping substrate specificity and are expressed in a highly tissueand species-selective manner. Corresponding to previously identified regulatory domain...
متن کاملExpression and characterization of functional dog flavin-containing monooxygenase 3.
Mammalian flavin-containing monooxygenase (FMO) enzymes catalyze oxidation at nucleophilic, heteroatom centers and are important for drug, xenobiotic, and endogenous substrate metabolism. In human liver, human FMO3 (hFMO3) is the most abundant FMO isoform and is known to contribute to the hepatic clearance of a variety of clinical drugs. The purpose of the current study was to express and compa...
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ژورنال
عنوان ژورنال: Free Radical Biology and Medicine
سال: 2019
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.09.038